耐酒石酸酸性磷酸酶(ACP5)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Acid Phosphatase 5, Tartrate Resistant (ACP5)
TRAP; TrATPase; Tartrate Resistant Acid Phosphatase; Type 5 acid phosphatase
- 编号SEA902Mu
- 物种Mus musculus (Mouse,小鼠) 相同的名称,不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围78-5,000pg/mL
- 灵敏度最小可检测剂量小于等于32pg/mL.
- 样本类型Serum, plasma, tissue homogenates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
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特异性
本试剂盒用于检测耐酒石酸酸性磷酸酶(ACP5),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的耐酒石酸酸性磷酸酶(ACP5)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。
样本 | 回收率范围(%) | 平均回收率(%) |
serum(n=5) | 87-94 | 90 |
EDTA plasma(n=5) | 85-94 | 90 |
heparin plasma(n=5) | 90-98 | 94 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%
线性
在定值血清及血浆样本内加入适量的耐酒石酸酸性磷酸酶(ACP5),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中耐酒石酸酸性磷酸酶(ACP5)含量的测定值与理论值的比率。
样本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 78-91% | 91-99% | 88-96% | 93-101% |
EDTA plasma(n=5) | 78-89% | 80-92% | 89-98% | 97-105% |
heparin plasma(n=5) | 86-104% | 98-105% | 92-104% | 93-101% |
稳定性
经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。
实验原理
将耐酒石酸酸性磷酸酶(ACP5)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的耐酒石酸酸性磷酸酶(ACP5)与连接于固相载体上的抗体结合,然后加入生物素化的耐酒石酸酸性磷酸酶(ACP5)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的耐酒石酸酸性磷酸酶(ACP5)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。
增值服务
相关产品
编号 | 适用物种:Mus musculus (Mouse,小鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPA902Mu01 | 耐酒石酸酸性磷酸酶(ACP5)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA902Mu02 | 耐酒石酸酸性磷酸酶(ACP5)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA902Mu02 | 耐酒石酸酸性磷酸酶(ACP5)多克隆抗体 | WB; IHC; ICC; IP. |
PAA902Mu01 | 耐酒石酸酸性磷酸酶(ACP5)多克隆抗体 | WB; IHC; ICC; IP. |
SEA902Mu | 耐酒石酸酸性磷酸酶(ACP5)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEA902Mu | 耐酒石酸酸性磷酸酶(ACP5)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA902Mu | 耐酒石酸酸性磷酸酶(ACP5)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
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