补体成分5a(C5a)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Complement Component 5a (C5a)
- 编号SEA388Mu
- 物种Mus musculus (Mouse,小鼠) 相同的名称,不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围1.56-100ng/mL
- 灵敏度最小可检测剂量小于等于0.57ng/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2419 ¥ 3456 ¥ 15552 ¥ 29376 ¥ 241920
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特异性
本试剂盒用于检测补体成分5a(C5a),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的补体成分5a(C5a)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。
样本 | 回收率范围(%) | 平均回收率(%) |
serum(n=5) | 82-103 | 93 |
EDTA plasma(n=5) | 80-95 | 92 |
heparin plasma(n=5) | 85-95 | 89 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%
线性
在定值血清及血浆样本内加入适量的补体成分5a(C5a),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中补体成分5a(C5a)含量的测定值与理论值的比率。
样本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 92-105% | 96-103% | 99-105% | 80-96% |
EDTA plasma(n=5) | 79-93% | 80-96% | 91-105% | 80-91% |
heparin plasma(n=5) | 86-94% | 86-98% | 83-102% | 89-102% |
稳定性
经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。
实验原理
将补体成分5a(C5a)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的补体成分5a(C5a)与连接于固相载体上的抗体结合,然后加入生物素化的补体成分5a(C5a)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的补体成分5a(C5a)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。
相关产品
编号 | 适用物种:Mus musculus (Mouse,小鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPA388Mu01 | 补体成分5a(C5a)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA388Mu62 | 补体成分5a(C5a)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA388Mu61 | 补体成分5a(C5a)活性蛋白 | Cell culture; Activity Assays. |
EPA388Mu61 | 补体成分5a(C5a)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA388Mu01 | 补体成分5a(C5a)多克隆抗体 | WB; IHC; ICC; IP. |
LAA388Mu71 | 补体成分5a(C5a)多克隆抗体(生物素标记) | WB; IHC; ICC. |
MAA388Mu21 | 补体成分5a(C5a)单克隆抗体 | WB; IHC; ICC; IP. |
LAA388Mu72 | 补体成分5a(C5a)单克隆抗体(生物素标记) | WB; IHC; ICC. |
SEA388Mu | 补体成分5a(C5a)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEA388Mu | 补体成分5a(C5a)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
HEA388Mu | 补体成分5a(C5a)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA388Mu | 补体成分5a(C5a)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA388Mu | 补体成分5a(C5a)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
PSA388Mu01 | 补体成分5a(C5a)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSA388Mu01 | 补体成分5a(C5a)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
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Journal of Neuroimmunology | Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis [Pubmed:27235358] |
International Immunopharmacology | Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery [Pubmed:26802602] |
Digital Repository | Dosagem de frações ativadas do sistema complemento em empiema induzido em ratos [10183] |
Environmental Pollution | Cadmium-induced immune abnormality is a key pathogenic event in human and rat models of preeclampsia [pubmed:27511439] |
Cancer Letters | Complement C5a/C5aR pathway potentiates the pathogenesis Q5 of gastric cancer by down-regulating p21 expression [pubmed:29031586] |
Acta Paediatrica | Serum complement factor 5a levels are associated with nonalcoholic fatty liver disease in obese children [DOI: 10.1111/apa.14106] |
Scientific Reports | Splenectomy modulates early immuno-inflammatory responses to trauma-hemorrhage and protects mice against secondary sepsis [Pubmed: 30291296] |
Nutrients | The Beneficial Effects of Lactobacillus plantarum PS128 on High-Intensity, Exercise-Induced Oxidative Stress, Inflammation, and Performance in Triathletes [Pubmed: 30736479] |
Journal of NeuroVirology | Intrathecal complement activation by the classical pathway in tick-borne encephalitis [Pubmed: 30850976] |
PLoS One | Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model [Pubmed: 31247004] |
Frontiers in Immunology | C5a/C5aR Pathway is Critical for the Pathogenesis of Psoriasis [Pubmed: 31447855] |
Phytomedicine | Novel evidence that an alternative complement cascade pathway is involved in optimal mobilization of hematopoietic stem/progenitor cells in Nlrp3 … [] |
Aging | C5aR deficiency attenuates the breast cancer development via the p38/p21 axis [Pubmed: 32669478] |
STEM CELL REVIEWS AND REPORTS | A Novel Evidence That Mannan Binding Lectin (MBL) Pathway of Complement Cascade Activation is Involved in Homing and Engraftment of Hematopoietic Stem … [Pubmed: 32406006] |
Modulation of C5a-C5aR interactions against murine mammary cancer cell line [] | |
FASEB J | C‐reactive protein inhibits C3a/C3aR‐dependent podocyte autophagy in favor of diabetic kidney disease [Pubmed:35503088] |